David Zappulla

Academic Titles: 
Research Associate
Position Title: 
Research Associate
410-955-3022

725 North Wolfe Street, PCTB 620

Baltimore, MD 21205
Research Interest: 
The telomerase RNA-protein complex, telomeres, and senescence.
Lab Members:
Namesort descending Classification Email
Alex Rittenhouse Undergraduate Student aritten5@jhu.edu
Evan P. Hass Graduate Student ehass1@jhu.edu
Selected Publications:
Hass, E.P. and Zappulla, D.C. (2017) A yeast two-hybrid system based on CRISPR-dCas9 for investigating RNA-protein interactions. bioRxiv doi: https://doi.org/10.1101/139600 [ link ]
Chen, H., Xue, J., Churikov, D., Hass, E.P., Lemon, L.D., Luciano, P., Bertuch, A.A., Zappulla, D.C., Geli, V., Wu, J., and Lei.M. Structural insights into yeast telomerase telomerase recruitment to telomeres. Cell (in press). 
Niederer, R.O., Papadopoulos, N., and Zappulla, D.C. (2016) Identification of novel noncoding transcripts in telomerase-negative yeast using RNA-seq. Scientific Reports 6, 19376; doi: https://doi.org/10.1038/srep19376 [ link ]
Mefford, M.A. and Zappulla, D.C. (2016) Physical connectivity mapping by circular permutation of human telomerase RNA reveals new regions critical for activity and processivity. Molecular and Cellular Biology 36:251–261. [ link ]
Hass, E. and Zappulla, D.C. (2015) The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae. eLIFE 2015; 10.7554/eLife.07750  [ link ]
Lebo, K.J., Niederer, R.O., and Zappulla, D.C. (2015) A second essential function of the Est1-binding arm of yeast telomerase RNA. RNA 21:862–76. [ link ]
Niederer, R.O. and Zappulla, D.C. (2015) Refined secondary structure models of the core of yeast and human telomerase RNAs directed by SHAPE. RNA 21:254–261. [ link ]