CRISPR'd worm: A C. elegans hermaphrodite glows green after insertion of GFP in a gene expressed in the germ line and in embryos. Homology-dependent repair of CRISPR-Cas9-induced breaks is very efficient in C. elegans: 33 bases of homology are sufficient to target GFP to gene of choice. Alex Paix and Geraldine Seydoux
Vasculature of the adult mouse retina
Stained with GS-lectin. Different depths are color-coded.
Centrosome number and genome instability
The rhomboid enzyme from E. coli
Mouse retina flat mount
Stained with GS lectin (red) and immunostained for smooth muscle actin (green). The image is at the vitreous surface of the retina; the optic disc is at the right.
The primary cilium
Human rhomboid enzyme localized by immunofluorescence in cultured cells
Neurofilaments visualized in whole-mount mouse embryo
Falciparum malaria parasites replicating within red blood cells
Smith J, Calidas D, Schmidt H, Lu T, Rasoloson D, Seydoux G. (2016) Spatial patterning of P granules by RNA-induced phase separation of the intrinsically-disordered protein MEG-3. eLife 2016;10.7554/eLife.21337. [ link ]
Wang F, Ranjan A, Wei D, Wu C. (2016) Comment on "A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme". Science 353(6297):358, PMID: 27463665. [ pdf ]
Lambrus, B.G., Daggubati, V., Uetake, Y., Scott, P.M., Clutario, K.M., Sluder, G., Holland, A.J. (2016) A USP28-53BP1-p53-p21 signaling axis arrests growth following centrosome loss or prolonged mitosis. Journal of Cell Biology 214:143-153. [ link ][ pdf ]